neurofibromatosis

cell line

mir-128-1

miRNA

up regulation

homo sapiens

RT-PCR; dual-lucimmunofluorescenceerase reporter assay; etc.

NA

NA

Neurofibromin 1 is a miRNA target in neurons

2012

"Using reporter assays, we provided evidence that mir-128 and to a lesser extent mir-137 and mir-103 reduced neurofibromin 1 reporter levels through specific binding to nf1 3-utr. Mutations in all three predicted binding sites eliminated the reporter response. Mir-128 and mir-137, unlike mir-103 that showed a more ubiquitous expression, were predominantly expressed in brain with a distribution that resembled neurofibromin 1 expression in different tissues as well as during the course of neuronal development. In the nervous system, all three micrornas showed highest expression in neurons and least in schwann cells and astrocytes. Overexpression of mir-128 alone or with mir-103 and mir-137 significantly reduced endogenous neurofibromin 1 protein levels, while antisense inhibition of these micrornas enhanced translation of endogenous neurofibromin 1 and reporter in primary cultures of hippocampal neurons."